Topic：High-Throughput T Cell Repertoire Profiling Enabled Systems Immunology and Immune Engineering

Time：July 18, at 16：30

Venue：Room 317, Yifu Scientific Center

Biography of the Speaker：

Dr. Jiang is an associate professor in the Department of Biomedical Engineering at the University of Texas at Austin. She obtained her Ph.D. from Georgia Institute of Technology where she developed a tool to measure T cell receptor affinity on T cell membrane and used it to study molecular mechanisms of T cell receptor antigenic ligand discrimination. Before joining the faculty at UT Austin in January 2012, she was a postdoctoral fellow in Dr. Stephen Quake’s lab at Stanford University where she developed the first generation of high-throughput immune repertoire sequencing technology. Her lab at UT Austin focuses on systems immunology by developing technologies that enable the direct profiling of human immune systems in cancer and vaccination. These technologies include a 2nd generation of immune repertoire sequencing technology that increase the accuracy 150 times, a high-throughput T cell receptor affinity and sequence test, and a high-throughput tool to link antigen specificity to T cell receptor sequences. Dr. Jiang is the recipient of the prestigious NIH Pathway to Independence Award (K99/R00), Cancer Prevention and Research Institute of Texas, Damon Runyon-Rachleff Innovator Award, and NSF CAREER Award.

Abstract：

Recent technological advancement has enabled high-throughput measurement of immune receptor sequence repertoire as well as characterizing of T cell phenotype and function at single cell level. However, how to evaluate T cell receptor binding affinity in a high-throughput fashion and decipher the interaction between the ligand repertoire and T cell receptor repertoire are still challenging. In this talk, I will first introduce three different technologies that my lab developed, an ultra-accurate immune repertoire sequencing, a high-throughput TCR affinity measurement, and a high-throughput linking of antigen specificity to T cell receptor sequences. Then, I will give examples on how to use them to study antibody repertoire development and its response to malaria infection in infants and profile T cell repertoire for cancer immunotherapy.

Beijing Advanced Innovation Center for Biomedical Engineering